high resolution spatial multiomics platforms  (Spatial Transcriptomics Inc)

Journal: MedComm

Article Title: Neuro‐Immune Crosstalk: Molecular Mechanisms, Biological Functions, Diseases, and Therapeutic Targets

doi: 10.1002/mco2.70497

Figure Lengend Snippet: Timeline of key milestones in neuroimmunology research. This timeline outlines major discoveries that have shaped the field of neuroimmunology. During the 1950s to 1970s, a series of foundational studies established the concept of “immune privilege” in the brain. Since the late 20th century, growing evidence has revealed bidirectional communication between the central nervous system (CNS) and the immune system, including active immune surveillance by the CNS and crucial regulatory roles of immune cells in neurogenesis and neural protection. In recent years, the integration of cutting‐edge technologies—such as single‐cell multiomics, tissue clearing, and organoid models—has enabled precise dissection of neuro‐immune interactions and facilitated the development of individualized interventions, ushering the field into a new era of mechanistic and translational research. Abbreviations : PNS, parasympathetic nervous system; SNS, sympathetic nervous system.

Article Snippet: To overcome these challenges, researchers have developed cutting‐edge tools, including: (1) high‐resolution spatial multiomics platforms (e.g., integrated spatial transcriptomics‐proteomics) for spatiotemporal mapping of neuro‐immune interactions to construct comprehensive “neuro‐immune connectomes”; (2) optimized organoid coculture systems, particularly brain–immune cell interaction models; and (3) advanced in vivo imaging techniques (e.g., two‐photon microscopy coupled with specific reporter systems) for real‐time observation of neuro‐immune processes.

Techniques: Dissection, Clinical Proteomics

Journal: MedComm

Article Title: Neuro‐Immune Crosstalk: Molecular Mechanisms, Biological Functions, Diseases, and Therapeutic Targets

doi: 10.1002/mco2.70497

Figure Lengend Snippet: Key technologies in neuroimmunology research. Advanced technologies hold great potential for deciphering neuro‐immune interactions, yet each category possesses distinct advantages and limitations. Single‐cell multiomics enables high‐resolution dissection of cellular heterogeneity but is costly and involves complex data analysis. Spatiotemporal and intravital imaging allows real‐time dynamic monitoring of cellular activities but is constrained by limited imaging depth and phototoxicity. Tissue clearing techniques facilitate 3D visualization of thick specimens but may compromise fluorescence signals and antigen integrity. Viral and synthetic biology tools provide precise genetic manipulation capabilities yet carry risks of immunogenicity and off‐target effects. Humanized and organoid models better recapitulate human physiology but often lack authentic microenvironmental contexts and involve complex culture systems. In vivo visualization and modulation technologies offer high physiological relevance, though invasive procedures and technical challenges remain substantial obstacles.

Article Snippet: To overcome these challenges, researchers have developed cutting‐edge tools, including: (1) high‐resolution spatial multiomics platforms (e.g., integrated spatial transcriptomics‐proteomics) for spatiotemporal mapping of neuro‐immune interactions to construct comprehensive “neuro‐immune connectomes”; (2) optimized organoid coculture systems, particularly brain–immune cell interaction models; and (3) advanced in vivo imaging techniques (e.g., two‐photon microscopy coupled with specific reporter systems) for real‐time observation of neuro‐immune processes.

Techniques: Dissection, Imaging, Fluorescence, Immunopeptidomics, In Vivo